Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton
| Autor: | Asmarani Kusumawati, Atik Ratnawati, Ida Arlita Wulandari, Sri Hartati, Tri Untari |
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| Jazyk: | English<br />Indonesian |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Jurnal Veteriner, Vol 16, Iss 4, Pp 569-575 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1411-8327 2477-5665 |
| Popis: | Jembrana disease is an infectious disease in Bali cattle cause by a member of lentivirus calledjembrana disease virus (JDV). It causes an acute and severe disease syndrome with short incubationperiod. As the disease has spread to several areas in Indonesia, a simple and rapid detection method isrequired. The objective of this study to apply rapid diagnostic method for JDVTabanan 1995 strain basedon Nucleic Acid Sequence Based Amplification (NASBA) methods targeting env-tm gene. The steps of thisresearch consisted of viral RNA isolation from organ and blood of cattle experimentaly infected withJDVTabanan 1995 strain . RNA amplification was conducted by NASBA using waterbath. The NASBAproducts were then separated on 2 % agarose gel. Using this technique JDV positive result was obtainedfrom organ samples such as spleen, liver, lung, prefemoralis lymph node, prescapularis lymph node andblood generating a RNA fragment of 207 bp. In this study, diagnosis method for env tm of JDV Tabanan1995 strain can be conducted by isothermal amplification NASBA. |
| Databáze: | Directory of Open Access Journals |
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