| Autor: |
Darshan V. Chaudhary, Daxesh P. Patel, Priyanka A. Shah, Jaivik V. Shah, Mallika Sanyal, Pranav S. Shrivastav |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Journal of Pharmaceutical Analysis, Vol 6, Iss 2, Pp 87-94 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2095-1779 |
| DOI: |
10.1016/j.jpha.2015.09.001 |
| Popis: |
An improved and reliable ultra-performance liquid chromatography/tandem mass spectrometry (UPLC–MS/MS) method has been developed and validated for the determination of lercanidipine in human plasma. Plasma samples with lercanidipine-d3 as an internal standard (IS) were prepared by solid phase extraction on Phenomenex Strata-X cartridges using 100 µL of human plasma. Chromatographic analysis was performed on UPLC BEH C18 (50 mm×2.1 mm, 1.7 µm) column under isocratic conditions. Linear calibration curves were obtained over a wide dynamic concentration range of 0.010–20.0 ng/mL. Matrix effect was assessed by post-column infusion, post-extraction spiking and standard-line slope methods. The mean extraction recovery was >94% for the analyte and IS. Inter-batch and intra-batch precision (% CV) across five quality controls was |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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