A High-Density Genetic Map for Cucumber (Cucumis Sativus L.) Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber

Autor: Wenying eZhu, Long eHuang, Long eChen, Jiantao eYang, Jiani eWu, Meiling eQu, Danqing eYao, Chunli eGuo, Hongli eLian, Huanle eHe, Junsong ePan, Run eCai
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Frontiers in Plant Science, Vol 7 (2016)
Druh dokumentu: article
ISSN: 1664-462X
DOI: 10.3389/fpls.2016.00437
Popis: High-density genetic linkage map plays an important role in genome assembly and QTL fine mapping. Since the coming of next-generation sequencing (NGS), makes the structure of high-density linkage maps much more convenient and practical, which simplifies SNP discovery and high-throughput genotyping. In this research, a high-density linkage map of cucumber was structured using specific length amplified fragment sequencing, using 153 F2 populations of S1000×S1002. The high-density genetic map composed 3,057 SLAFs, including 4,475 SNP markers on 7 chromosomes, and spanned 1061.19cM. The average genetic distance is 0.35cM. Based on this high-density genome map, QTL analysis was performed on two cucumber fruit traits, fruit length and fruit diameter. There are 15 QTLs for the two fruit traits were detected.
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