| Autor: |
Ling-Chun Kong, Hong Zou, Jing-Jing Li, Yun Ling, Hui-Xin Tang |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Guoji Yanke Zazhi, Vol 23, Iss 1, Pp 17-22 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1672-5123 |
| DOI: |
10.3980/j.issn.1672-5123.2023.1.04 |
| Popis: |
AIM: To investigate the protective effect of Modified Taohong Siwu Decoction(MTSD)on hypoxia injury to retinal Müller cells rMC-1.METHODS: Retinal Müller cells rMC-1 were interfered with the MTSD drug-containing serum under hypoxia condition, and were randomly divided into control group(21%O2), hypoxia model group(1%O2), MTSD drug-containing serum low-dose(1%O2+5% medicated serum), medium-dose(1%O2+10% medicated serum)and high-dose(1%O2+15%medicated serum)groups. Cell viability was detected by CCK-8 method, and secretion of vascular endothelial growth factor(VEGF)and pigment epithelium derived factor(PEDF)was detected by ELISA. The protein expressions of p-STAT3, STAT3 and hypoxia-inducible factor-1α(HIF-1α)were detected by Western blot, and the gene expressions of VEGF, PEDF, STAT3 and HIF-1α were detected by real-time polymerase chain reaction(PCR).RESULTS: The viability of rMC-1 cells was significantly inhibited when cultured at 1%O2 for 48h compared with that of control group(P0.05). The low and medium doses of MTSD could reduce the protein expressions of VEGF in supernatant of rMC-1 cells under hypoxia condition(P0.05). The above two dose groups down-regulated the protein levels of both p-STAT3 and HIF-1α(P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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