Differentiation of Dental Pulp Stem Cells into Neuron-Like Cells in Serum-Free Medium
| Autor: | Shahrul Hisham Zainal Ariffin, Shabnam Kermani, Intan Zarina Zainol Abidin, Rohaya Megat Abdul Wahab, Zulham Yamamoto, Sahidan Senafi, Zaidah Zainal Ariffin, Mohamad Abdul Razak |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Stem Cells International, Vol 2013 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1687-966X 1687-9678 |
| DOI: | 10.1155/2013/250740 |
| Popis: | Dental pulp tissue contains dental pulp stem cells (DPSCs). Dental pulp cells (also known as dental pulp-derived mesenchymal stem cells) are capable of differentiating into multilineage cells including neuron-like cells. The aim of this study was to examine the capability of DPSCs to differentiate into neuron-like cells without using any reagents or growth factors. DPSCs were isolated from teeth extracted from 6- to 8-week-old mice and maintained in complete medium. The cells from the fourth passage were induced to differentiate by culturing in medium without serum or growth factors. RT-PCR molecular analysis showed characteristics of Cd146+, Cd166+, and Cd31− in DPSCs, indicating that these cells are mesenchymal stem cells rather than hematopoietic stem cells. After 5 days of neuronal differentiation, the cells showed neuron-like morphological changes and expressed MAP2 protein. The activation of Nestin was observed at low level prior to differentiation and increased after 5 days of culture in differentiation medium, whereas Tub3 was activated only after 5 days of neuronal differentiation. The proliferation of the differentiated cells decreased in comparison to that of the control cells. Dental pulp stem cells are induced to differentiate into neuron-like cells when cultured in serum- and growth factor-free medium. |
| Databáze: | Directory of Open Access Journals |
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