Protein Adsorption on Hybrids of Thermoresponsive Polymers and Single-Walled Carbon Nanotubes

Autor: Kazuo Umemura, Katsuki Izumi, Yoshikazu Kumashiro, Shusuke Oura, Teruo Okano
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: International Journal of Polymer Science, Vol 2016 (2016)
Druh dokumentu: article
ISSN: 1687-9422
1687-9430
DOI: 10.1155/2016/3539609
Popis: Poly(N-isopropylacrylamide) (PNIPAAm) is one of the most popular thermoresponsive polymers. Adsorption of RecA proteins onto hybrids of PNIPAAm and single-walled carbon nanotubes (SWNTs) was observed in the presence and absence of DNA molecules. Although RecA molecules were adsorbed efficiently onto the hybrid surfaces at 37°C, even in the absence of DNA molecules, the adsorption of RecA was inhibited at 4°C. These results suggest that the thermoresponsive functions of PNIPAAm were effective, even on the SWNT surfaces, which supports the possibility of developing nanobiodevices using PNIPAAm-SWNT hybrids. However, although RecA is a DNA binding protein, there was no significant difference in the adsorption of RecA onto PNIPAAm-SWNT surfaces with and without DNA molecules. This study provides fundamental information for potential biological applications of PNIPAAm-SWNT hybrids.
Databáze: Directory of Open Access Journals
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