Effect of Propofol on Sevoflurane-Induced Myocardial Preconditioning in the Experiment
| Autor: | K. Yu Borisov, V. V. Moroz, O. A. Grebenchikov, E. Yu, Plotnikov, D. I. Levikov, R. A. Cherpakov, V. V. Likhvantsev |
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| Jazyk: | English<br />Russian |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Общая реаниматология, Vol 9, Iss 4 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1813-9779 2411-7110 |
| DOI: | 10.15360/1813-9779-2013-4-30 |
| Popis: | Objective: to estimate the magnitude of the cardioprotective properties of sevoflurane and propofol, when they are used alone and in combination in the acute experiment on rats after total ischemia/reperfusion (I/R). Materials and methods. The investigation was conducted on 50 male albino outbred rats weighing 202±19 g (M±ff), by using a total I/R model. The animals were randomized into 5 groups: 1) intraperitoneal chloral hydrate 300 mg/kg; 15-minute mechanical ventilation (MV); 2) inhalation induction with sevoflurane in an exsiccator; MV; injection of about 2—2.5 MAC sevoflurane in the expiratory breathing circuit for 15 minutes — anesthetic preconditioning simulation (APS); 3) anesthesia and APS with sevoflurane; then 10-minute ischemia simulation, followed by resuscitation and 5-minute reperfusion; 4) chloral hydrate anesthesia; injection of propofol 2.5 mg/kg in the right internal jugular vein; APS simulation; 5) chloral hydrate anesthesia; injection of propofol 2.5 mg/kg in the right internal jugular vein; and simulation of APS with sevoflurane. The hearts were extracted from all the animals and homogenized for further examination. The concentrations of total and phosphorylated glycogen synthase kinase-3/8 (GSK-3/8 and pGSK-3/8, respectively) were estimated by Western blotting. Results. The concentration of total GSK-3/8 was comparable in the groups. Group 2 showed a 1.5-fold increase in pGSK-3^ concentrations (p |
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