| Autor: |
Shaowei Ding, Benjamin J. Brownlee, Kshama Parate, Cicero C. Pola, Bolin Chen, Jesse M. Hostetter, Douglas Jones, John Jackman, Brian D. Iverson, Jonathan C. Claussen |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Global Challenges, Vol 8, Iss 9, Pp n/a-n/a (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2056-6646 |
| DOI: |
10.1002/gch2.202400021 |
| Popis: |
Abstract Highly sensitive vertically aligned carbon nanotube arrays (VANTAs) interdigitated electrode (IDE) arrays are developed for electrochemical biosensing of two cytokines (i.e., interleukin‐10 (IL‐10) and interferon‐gamma (IFN‐γ)) that are useful for early detection Johne's disease (Bovine Paratuberculosis) in cattle. The high aspect ratio VANTA‐IDEs (50–60 µm in height) are grown through a chemical vapor deposition process from an iron (Fe) catalyst that is lithographically patterned on a silicon wafer with equal finger width and inter‐finger spacing of 25 µm. After functionalization with distinct antibodies the VANTA‐IDEs are capable of selective detection of both IL‐10 and IFN‐γ within an actual biological matrix (i.e., diluted bovine implant supernatant) over concentration ranges of 0.1 to 30 pg mL−1 (limit of detection – LOD: 0.0911 pg mL−1) and 50–500 pg mL−1 (LOD: 24.17 pg mL−1), respectively with a response time of |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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