| Autor: |
P.C. Yap, H.Y. Lee, L.C. Chai, S. Abubakar |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
International Journal of Infectious Diseases, Vol 116, Iss , Pp S81-S82 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1201-9712 |
| DOI: |
10.1016/j.ijid.2021.12.193 |
| Popis: |
Purpose: Listeria monocytogenes is highly abundant in nature as a saprophyte and capable to utilize a variety of carbon sources, mainly derived from decaying plants. The transcriptional activator regulator (PrfA) that mediates the expression of virulence genes, is inactive in the presence of nutrient rich sources including nature environment and laboratory culture media. However, PrfA activity is highly induced in host cell and caused listeriosis, suggesting PrfA activity is controlled by the nutrient availability in their surroundings. This study is to identify the key nutrient that induce PrfA activity in the host cell. Methods & Materials: L. monocytogenes ATCC19111 (hemolytic) and L. monocytogenes ATCC15313 (non-hemolytic) were selected to profile the metabolic differences. Four Phenotype MicroArray assay (PM1 to PM4) including carbon, nitrogen and sulphur-phosphorus assay were performed to profile their phenotype characteristics. Phenotype MicroArray assay was validated though determining the growth of L. monocytogenes at optical density of 600nm (OD600). Results: There are significant metabolism differences among haemolytic and non-haemolytic L. monocytogenes, particularly in carbon metabolism. The shared carbon sources by both L. monocytogenes are mainly phosphotransferase system (PTS) sugar, suggesting PTS sugar is not involving in L. monocytogenes pathogenesis. Interestingly, the rate of respiration of L. monocytogenes ATCC15313 is significantly higher than L. monocytogenes. Two potential carbon sources namely L-serine and D-Gluconic acid were found to be potentially involved in L. monocytogenes pathogenesis as they are only metabolized by L. monocytogenes ATCC19111. D-Gluconic acid is metabolized by L. monocytogenes through pentose phosphate pathway where the intermediates are involved in nucleotide synthesis that could possibly form amino acid. Besides, L-serine metabolism is involved in amino acid metabolism pathway and produced other amino acids as intermediates, suggesting the amino acids are involved in L. monocytogenes pathogenesis. Conclusion: Phenotype MicroArray is a simple and bioinformative technology for physiological differences determination in different bacteria strains. L. monocytogenes metabolized carbon sources readily compared with other sources. L-serine and D-Gluconic acid as carbon sources were found to be potential in pathogenesis of L. monocytogenes. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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