A system for the study of roots 3D kinematics in hydroponic culture: a study on the oscillatory features of root tip

Autor: Valentina Simonetti, Laura Ravazzolo, Benedetto Ruperti, Silvia Quaggiotti, Umberto Castiello
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Plant Methods, Vol 20, Iss 1, Pp 1-10 (2024)
Druh dokumentu: article
ISSN: 1746-4811
DOI: 10.1186/s13007-024-01178-3
Popis: Abstract Background The root of a plant is a fundamental organ for the multisensory perception of the environment. Investigating root growth dynamics as a mean of their interaction with the environment is of key importance for improving knowledge in plant behaviour, plant biology and agriculture. To date, it is difficult to study roots movements from a dynamic perspective given that available technologies for root imaging focus mostly on static characterizations, lacking temporal and three-dimensional (3D) spatial information. This paper describes a new system based on time-lapse for the 3D reconstruction and analysis of roots growing in hydroponics. Results The system is based on infrared stereo-cameras acquiring time-lapse images of the roots for 3D reconstruction. The acquisition protocol guarantees the root growth in complete dark while the upper part of the plant grows in normal light conditions. The system extracts the 3D trajectory of the root tip and a set of descriptive features in both the temporal and frequency domains. The system has been used on Zea mays L. (B73) during the first week of growth and shows good inter-reliability between operators with an Intra Class Correlation Coefficient (ICC) > 0.9 for all features extracted. It also showed measurement accuracy with a median difference of
Databáze: Directory of Open Access Journals
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