Impact of IL-12 and IL-18 Combined Stimulation on INF-γ and TNF-α Production by PBMCs Derived from Egyptian Ovarian Cancer Patients
| Autor: | Nourhan E. Mohamed, Noha A. Mahana, Abeer Mahmoud Badr, Ola S. Ahmed, Ahmed M. Lymona, Sarah S. Nasr |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | International Journal of Biomedicine, Vol 14, Iss 3, Pp 435-400 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2158-0510 2158-0529 |
| DOI: | 10.21103/Article14(3)_OA7 |
| Popis: | Background: Cytokines play a crucial role in modulating the immune cells' response to tumors. Utilizing IL-18 and IL-12 as immunotherapeutic agents has gained much attention for cancer treatment. This study aimed to investigate the effect of combined stimulation with IL-12 and IL-18 on the ability of peripheral blood mononuclear cells (PBMCs) derived from Egyptian ovarian cancer (OC) patients to produce IFN-γ and TNF-α. Methods and Results: This in vitro study included 8 patients with benign ovarian tumors, 8 patients with low-grade OC, and 8 patients with high-grade OC. PBMCs were stimulated with IL-12 (10ng/ml) and IL-18 (50ng/ml) for 7 days. The levels of IFN-γ and TNF-α were quantified before and after stimulation by quantitative sandwich ELISA. Stimulated PBMCs isolated from patients with low-grade OC showed good ability to produce high levels of IFN-γ, while stimulated PBMCs isolated from patients with high-grade OC patients showed good and high production of TNF-α. Conclusion: We found that the combined stimulation with IL-12 and IL-18 significantly enhances the production of IFN-γ and TNF-α by PBMCs in OC patients, suggesting a potential avenue for cancer immunotherapy. |
| Databáze: | Directory of Open Access Journals |
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