Populations of Eimeria tenella express resistance to commonly used anticoccidial drugs in southern Nigeria

Autor: Agatha E. Ojimelukwe, Deborah E. Emedhem, Gabriel O. Agu, Florence O. Nduka, Austin E. Abah
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: International Journal of Veterinary Science and Medicine, Vol 6, Iss 2, Pp 192-200 (2018)
Druh dokumentu: article
ISSN: 2314-4599
DOI: 10.1016/j.ijvsm.2018.06.003
Popis: Coccidiosis is one of the most economically important diseases of poultry. This study determined the preponderance of chicken Eimeria in southern Nigeria and assessed the parasite’s resistance to three anticoccidial drugs: Amprolium hydrochloride; Amprolium hydrochloride + Sulfaquinoxaline-Sodium; and Toltrazuril. Multiplex PCR amplification of the SCAR region was used to confirm Eimeria preponderance. Resistance was assessed following the inoculation of 2.32 × 105 infective oocysts into broilers. Data on weight gain, feed intake, feed conversion and fecal oocyst shed were recorded. At 7 days post inoculation 9 birds per treatment were sacrificed and assessed for macroscopic lesions in four intestinal regions. Percent optimum anticoccidial activity (POAA), Anticoccidial index (ACI) and Anticoccidial sensitivity test (AST) were used to access resistance. The preponderance of Eimeria spp. were E. tenella (77%), E. necatrix (55%), E. acervulina (44%) and E. mitis (11%), with multi-species infection occurring in 55% of samples assessed. Fecal oocyst shedding was low (P
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