| Autor: |
Jéssica de Santana Brito, Amanda de Oliveira Marinho, Leydianne Leite de Siqueira Patriota, Wyndly Daniel Cardoso Gaião, Diego José Lira Torres, Patrícia Maria Guedes Paiva, Virgínia Maria Barros de Lorena, Cláudio Gabriel Rodrigues, Márcia Bezerra da Silva, Thiago Henrique Napoleão |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Macromol, Vol 3, Iss 2, Pp 290-302 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2673-6209 |
| DOI: |
10.3390/macromol3020018 |
| Popis: |
Lectins (carbohydrate-binding proteins) are able to distinguish different patterns of glycosylation on cell surfaces. This study investigated the effects of lectins from Alpinia purpurata inflorescence (ApuL) and Schinus terebinthifolia leaf (SteLL) on the viability of human leukemia cells (K562, chronic myeloid leukemia; JURKAT, acute lymphoblastic leukemia) and mesenchymal stem cells (MSCs) from human umbilical cords. In addition, possible immunomodulatory effects of ApuL and SteLL on MSCs were assessed by determining cytokine levels in cultures. ApuL reduced the viability of JURKAT cells (IC50: 12.5 μg/mL), inducing both apoptosis and necrosis. For K562 cells, ApuL at 50 µg/mL caused a decrease in viability, but of only 8.8%. Conversely, SteLL exerted a cytotoxic effect on K562 (IC50: 6.0 μg/mL), inducing apoptosis, while it was not cytotoxic to JURKAT. ApuL and SteLL (0.19–100 μg/mL) did not decrease MSCs viability. Treatment with ApuL strongly suppressed (99.5% reduction) the release of IL-6 by MSCs. SteLL also reduced the levels of this cytokine in culture supernatant. In conclusion, ApuL and SteLL showed potential to reduce the viability of leukemia cells, as well as immunomodulatory effect on MSCs without being toxic to them. These biological properties can be explored biomedically and biotechnologically in the future. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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