| Autor: |
Tatsushi Yuri, Rosina Degrieck, Dagmara Minczakiewicz, Hideo Sato, Jo Kamada, Takuya Nakazawa, Ina Vandenbroucke, Katsumi Aoyagi, Hisashi Nojima |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Exploration of Neuroscience, Vol 2, Iss 5, Pp 238-244 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2834-5347 |
| DOI: |
10.37349/en.2023.00024 |
| Popis: |
Aim: Apolipoprotein E (ApoE) isoforms, especially the ApoE4 isoform, are genetic risk factors for Alzheimer’s disease (AD). Moreover, the APOE ε4 haplotype has a dose-dependent association with an increased risk of amyloid-related imaging abnormalities (ARIA) in individuals receiving disease-modifying therapy for AD. Therefore, the importance of APOE genotyping or proteotyping has been highlighted. Here, the authors developed fully automated chemiluminescence enzyme-immunoassay kit for ApoE4 and Pan-ApoE, and evaluated their diagnostic concordance with the APOE genotyping. Methods: One hundred seventy-eight specimens were analyzed using the Lumipulse® G ApoE4 and Pan-ApoE for the ApoE proteotype and evaluated its diagnostic concordance with the APOE genotype. Results: The ApoE4 kit specifically detected the ApoE4 concentration in plasma samples, and the polymorphism could be classified clearly by the ratio of ApoE4 and Pan-ApoE amount in plasma. Conclusions: The combination of Pan-ApoE and ApoE4-specific chemiluminescent enzyme immunoassay (CLEIA) assay is useful for predicting APOE ε4 allele status. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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