| Autor: |
Emily B. Hill, Bridget A. Baxter, Brigitte Pfluger, Caroline K. Slaughter, Melanie Beale, Hillary V. Smith, Sophia S. Stromberg, Madison Tipton, Hend Ibrahim, Sangeeta Rao, Heather Leach, Elizabeth P. Ryan |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Frontiers in Gastroenterology, Vol 2 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2813-1169 |
| DOI: |
10.3389/fgstr.2023.1087056 |
| Popis: |
IntroductionDietary intake of whole grains and legumes and adequate physical activity (PA) have been associated with reduced colorectal cancer (CRC) risk. A single-blinded, two-arm, randomized, placebo-controlled pilot trial was implemented to evaluate the impact of a 12-week dietary intervention of rice bran + navy bean supplementation and PA education on metabolite profiles and the gut microbiome among individuals at high risk of CRC.MethodsAdults (n=20) were randomized 1:1 to dietary intervention or control. All participants received PA education at baseline. Sixteen study foods were prepared with either heat-stabilized rice bran + navy bean powder or Fibersol®-2 as a placebo. Intervention participants consumed 30 g rice bran + 30 g navy bean powder daily; those in the control group consumed 10 g placebo daily. Non-targeted metabolite profiling was performed by UPLC-MS/MS to evaluate plasma, urine, and stool at 0, 6, and 12 weeks. Stool was also analyzed for primary and secondary bile acids (BAs) and short chain fatty acids (SCFAs) by UPLC-MS/MS and microbial community structure via 16S amplicon sequencing. Two-way ANOVA was used to compare differences between groups for metabolites, and mixed models were used to compare differences between groups for BAs, SCFAs, and alpha and beta diversity measures of microbial community structure.ResultsAcross biological matrices, the intervention resulted in changes to several amino acid and lipid metabolites, compared to control. There was a 2.33-fold difference in plasma (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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