Validação de método para determinação de ácidos orgânicos voláteis em efluentes de reatores anaeróbios empregando cromatografia líquida
Autor: | Maristela Barnes Rodrigues Cerqueira, Adriana Neves Dias, Sergiane Souza Caldas, Fabrício Butierres Santana, Marcelo Gonçalves Montes D'Oca, Ednei Gilberto Primel |
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Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
Rok vydání: | 2011 |
Předmět: | |
Zdroj: | Química Nova, Vol 34, Iss 1, Pp 156-159 (2011) |
Druh dokumentu: | article |
ISSN: | 1678-7064 0100-4042 |
DOI: | 10.1590/S0100-40422011000100029 |
Popis: | This work deals with the method validation for the determination of acetic, propionic and butyric acids (VFAs) in wastewaters from anaerobic reactors by HPLC-DAD. Separation was performed using a C18 column and the mobile phase composition were water pH 3.0 and methanol 90:10 (v/v). The detection and quantification was carried out at 220 nm. The method shows good linearity (r²>0.996), with adequate accuracy (89-102%) and relative standard deviations lower than 18%. The matrix effect was considered low (-4.1, -3.9 and 1.4%). The developed method is fast, simple and cheap; and it was applied in wastewater samples from anaerobic reactor. |
Databáze: | Directory of Open Access Journals |
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