Propolis nanoparticles synthesis and characterization with cytotoxic and apoptotic effects on breast cancer cells

Autor: Ebru Nur Ay, Armağan Caner, Ceylan Özsoy Hepokur, Ferdane Danişman Kalindemirtaş, Güneş Özen Eroğlu, İ. Afşin Kariper
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Journal of Taibah University for Science, Vol 17, Iss 1 (2023)
Druh dokumentu: article
ISSN: 16583655
1658-3655
DOI: 10.1080/16583655.2023.2249628
Popis: The aim of this work was to investigate the antiproliferative and pro-apoptotic effects of propolis on human breast cancer cells using a simple and inexpensive nanoformulation. Various methods were used to characterize the surface and morphology of propolis nanoparticles (NP-Pro), including UV-VIS, FTIR, DLS, and EDX-SEM. The cytotoxic activity of MCF-7, MDA-MB-231 breast cancer cells was measured by XTT technique and compared with healthy cells (MCF-10A). The apoptotic effect of NP-Pro was assessed by flow cytometry using Annexin/PI. The IC50 values of NP-Pro on MCF-7, MDA-MB-231 and MCF-10A cells were 13.67 ± 0.89, 17.89 ± 0.6, 29.9 ± 0.56 µg, respectively. According to these findings, the NP-Pro form we prepared was more cytotoxic on cancer cells than propolis. Cancer cells had much stronger apoptotic activity than healthy cells (p
Databáze: Directory of Open Access Journals
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