| Autor: |
Amvrosios Orfanidis, Nikolaos Raikos, Evdokia Brousa, Eleni Zangelidou, Orthodoxia Mastrogianni |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Separations, Vol 8, Iss 8, p 117 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2297-8739 |
| DOI: |
10.3390/separations8080117 |
| Popis: |
Forensic toxicologists often detect lidocaine in the biological fluids of the deceased, due to cardiopulmonary resuscitation (CPR) attempts prior to death. Here, we describe the development of a rapid, sensitive and robust method for the detection of lidocaine in postmortem whole blood using liquid−liquid extraction (LLE) followed by GC/MS analysis. The method showed a dynamic linear range of 100 to 6000 ng/mL with a linearity expressed by the regression coefficient (R2) and a value of 0.9947. The quantitation limit (LOQ) was found to be 0.03 ng/mL and the detection limit (LOD) 0.01 ng/mL. Recovery accuracy and repeatability were satisfactory. Finally, the method was applied to 23 real whole blood samples from cases where CPR was attempted. Blood concentrations ranged from 0.21–0.96 μg/mL. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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