Tropism of adeno-associated virus serotypes in mouse lungs via intratracheal instillation

Autor: Haoyu Wu, Ailing Zhao, Ye Bu, Weiping Yang, Lang He, Yujian Zhong, Dong Yao, Huapeng Li, Wenguang Yin
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Virology Journal, Vol 21, Iss 1, Pp 1-17 (2024)
Druh dokumentu: article
ISSN: 1743-422X
DOI: 10.1186/s12985-024-02575-9
Popis: Abstract Background Gene therapy holds great potential for treating various acquired and inherited pulmonary diseases. Adeno-associated viral (AAV) vectors have been thought to be primary candidates for gene delivery in patients with pulmonary diseases. However, the tropism of AAVs in the lungs remains largely unknown. Results Here, we investigate the tropism of twenty serotypes of AAVs by examining AAV-packed vector expression of the enhanced green fluorescent protein (eGFP) in mice. AAV1, AAV4, AAV5, AAV6, AAV6.2, AAV-PHP.B, and AAV-PHP.S exhibit high transduction rates in the airway epithelium. AAV1, AAV4, AAV5, AAV6, and AAV6.2 highly infect club cells. AAV1, AAV4, AAV5, AAV6, AAV6.2, and AAV-PHP.B efficiently infect ciliated cells. AAV8 and AAVrh10 can infect a few alveolar type I cells. AAV1, AAV5, AAV6, AAV6.2, AAV9, and AAVie can infect alveolar type II cells. AAV1, AAV5, AAVie, AAV-PHP.B, AAV-PHP.eB, and AAV-PHP.S can infect a few endothelial cells. However, none of these AAVs can efficiently infect neuroendocrine or smooth muscle cells. Conclusions Our findings provide comprehensive information about the tropism of AAVs in pulmonary epithelium in mice, which might be helpful in developing efficient AAV-mediated gene therapy strategies for pulmonary disease treatment.
Databáze: Directory of Open Access Journals
Nepřihlášeným uživatelům se plný text nezobrazuje
načítá se...