Comparative Metabarcoding and Metatranscriptomic Analysis of Microeukaryotes Within Coastal Surface Waters of West Greenland and Northwest Iceland

Autor: Stephanie Elferink, Sylke Wohlrab, Stefan Neuhaus, Allan Cembella, Lars Harms, Uwe John
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Frontiers in Marine Science, Vol 7 (2020)
Druh dokumentu: article
ISSN: 2296-7745
DOI: 10.3389/fmars.2020.00439
Popis: Climate change alters environmental conditions that are expected to have a profound effect on the biodiversity, community composition, and metabolic processes of microeukaryotic plankton in Arctic and Subarctic coastal waters. The molecular biodiversity [large subunit (LSU) rRNA gene] of three plankton size-fractions (micro-, nano-, and picoplankton) from coastal waters of ice-influenced west Greenland was compared with fractions from ice-free northwest Iceland within their summer environmental context. Putative metabolic functions were determined by differentially expressed mRNA (metatranscriptomics) of the microplankton. Temperature and salinity variations were more closely correlated than inorganic macronutrients with metabolic functions and community composition. Temperature explained much of the community variance, approximately 20% among micro- and nanoplankton, whereas other environmental factors accounted for rather low fractional variance (
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