Characterization of the phospholipid and fatty acid composition of Sendai virus.

Autor: J A Barnes, D J Pehowich, T M Allen
Jazyk: angličtina
Rok vydání: 1987
Předmět:
Zdroj: Journal of Lipid Research, Vol 28, Iss 2, Pp 130-137 (1987)
Druh dokumentu: article
ISSN: 0022-2275
DOI: 10.1016/S0022-2275(20)38714-9
Popis: The lipid composition of Sendai virus, propagated in chicken eggs, was analyzed by high performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and gas-liquid chromatography (GLC). Phosphatidylcholine was found to be the dominant phospholipid (37.3%) with phosphatidylethanolamine (26.8%) and phosphatidylserine (12.0%) also present in significant amounts. Analysis of the fatty acid methyl esters revealed that the dominant fatty acids in total phospholipid were: C16:0 (17.6%), C18:0 (15.4%), C18:1 (n-9) (22.0%), and C24:0 (6.0%). Cardiolipin, phosphatidylserine, and sphingomyelin contained higher levels of saturated fatty acids relative to phosphatidylinositol, phosphatidylethanolamine, and phosphatidylcholine.
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