| Autor: |
Marthe S. Christie, Mikko Laitaoja, Aasne K. Aarsand, Juha P. Kallio, Helene J. Bustad |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
FEBS Open Bio, Vol 12, Iss 12, Pp 2136-2146 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2211-5463 |
| DOI: |
10.1002/2211-5463.13490 |
| Popis: |
Hydroxymethylbilane synthase (HMBS) is the third enzyme involved in haem biosynthesis, in which it catalyses the formation of tetrapyrrole 1‐hydroxymethylbilane (HMB). In this process, HMBS binds four consecutive substrate molecules, creating the enzyme‐intermediate complexes ES, ES2, ES3 and ES4. Pathogenic variants in the HMBS gene are associated with the dominantly inherited disorder acute intermittent porphyria. In this study, we have characterised the p.R26H variant to shed light on the role of Arg26 in the elongation mechanism of HMBS and to provide insights into its effect on the enzyme. With selected biophysical methods, we have been able to show that p.R26H forms a single enzyme‐intermediate complex in the ES2‐state. We were also able to demonstrate that the p.R26H variant results in an inactive enzyme, which is unable to produce the HMB product. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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