DETERMINATION OF RUTIN IN LEAVES OF SALIX TRIANDRA USING THE METHOD OF PLANAR CHROMATOGRAPHY
| Autor: | E. G. Sannikova, T. D. Mezenova |
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| Jazyk: | ruština |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Фармация и фармакология (Пятигорск), Vol 4, Iss 1(14), Pp 85-90 (2016) |
| Druh dokumentu: | article |
| ISSN: | 2307-9266 2413-2241 |
| DOI: | 10.19163/2307-9266-2016-4-1(14)-85-90 |
| Popis: | The aim of the study is to develop new methods of identification and quantitative determination of rutin in the leaves of Salix triandra L. with thin-layer chromatography. The digital processing of the chromatograms was performed using «Sorbfil Videodensitometer» computer program (Krasnodar). For the chromatography analysis we used plates «Sorbfil» PTSH-P-A-UV, mobile phase: n-butanol-glacial acetic acid-water (4:1:1), detecting reagent – ammonia vapors. The rutin is detected as yellow spots with Rf 0.64±0.02. The detection limit is 0.5 μg/μl. The regression equation has the form: S=4.75×103 m. With the use of this method we found that the composition of rutin in leaves of Salix triandra was in the range 1.81±0.06% on the air-dry raw material (relative standard deviation RSD%=2.5%; the measurement error E=3.12%). The method is sensitive, linear, has a small error, and does not require expensive equipment. |
| Databáze: | Directory of Open Access Journals |
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