PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA

Autor: Yohei Ishibashi, Hatsumi Goda, Rie Hamaguchi, Keishi Sakaguchi, Takayoshi Sekiguchi, Yuko Ishiwata, Yuji Okita, Seiya Mochinaga, Shingo Ikeuchi, Takahiro Mizobuchi, Yoshitake Takao, Kazuki Mori, Kosuke Tashiro, Nozomu Okino, Daiske Honda, Masahiro Hayashi, Makoto Ito
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Communications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
Druh dokumentu: article
ISSN: 2399-3642
DOI: 10.1038/s42003-021-02857-w
Popis: Yohei Ishibashi and co-workers report that Parietichytrium sp. synthesizes DHA only via the elongase/desaturase pathway. They then engineered this thraustochytrid to produce EPA and n-3DPA by disrupting C20ELO and delta4 DES genes, respectively, in this biosynthetic pathway, suggesting that this may be a suitable system for the large scale production of these omega-3 fatty acids.
Databáze: Directory of Open Access Journals