Molecular and Serological Techniques to Determine the Acute and Chronic Phase of Toxoplasmosis in HIV Patients
| Autor: | F Shokri, R Abbaszadeh, M Rrostamnezhad, S Heydari digeh sara, N Marandi, A Bairami kuzeh konan, M Abolhasani, MJ Gharavi |
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| Jazyk: | English<br />Persian |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 21, Iss 1, Pp 140-146 (2019) |
| Druh dokumentu: | article |
| ISSN: | 1561-4107 2251-7170 |
| Popis: | BACKGROUND AND OBJECTIVE: Toxoplasma gondii is associated with several complications including neurological problems, ocular damage and encephalitis in immunodeficiency individuals. Early diagnosis of this infection can lead to better management of this disease. Therefore, this study was conducted to determine the presence of Toxoplasma gondii with two serologic and molecular methods in HIV-infected individuals. METHODS: In this cross-sectional study, 102 male patients with HIV with a mean age of 40 ± 9.2 years were examined. The serum sample was used for ELISA to determine the acute and chronic phase and cellular samples using Real Time-PCR for determining the acute phase of the disease. The relationship between age groups and the HIV transmission pathway, as well as the age group, was compared with the results of the Toxoplasma gondii test. FINDINGS: Out of 102 samples tested for IgM anti-Toxoplasma gondii antigen by ELFA, all (100%) samples were negative, but for anti-IgG anti-parasite, 44 samples (43.1%) were positive and 58 Sample (56.9%) was negative. Out of 102 samples tested by RT-PCR, all (100%) samples were negative for Toxoplasma DNA. There was a statistically significant relationship between age groups and transmission pathways (p |
| Databáze: | Directory of Open Access Journals |
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