| Autor: |
Kunxian YANG, Jiashu CHEN, Bingbing ZHAI, Weiming CHEN, Huanglan YANG, Yufen XIAO, Juping WANG, Wenjie MEI |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Journal of Holistic Integrative Pharmacy, Vol 2, Iss 4, Pp 261-269 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2707-3688 |
| DOI: |
10.1016/S2707-3688(23)00070-5 |
| Popis: |
Objective: To synthesize copper (II) porphyrin complexes and study their interactions with c-myc G-quadruplex DNA. Methods: The 5-p-hydroxyphenyl-10, 15, 20-tris (p-methoxyphenyl) copper (II) porphyrin complex [p-HTMOPPCu (II)] was synthesized by the conventional heating method. Ultraviolet (UV) titration, fluorescence titration, fluorescence resonance energy transfer (FRET) melting point and competitive assays were used to study the interactions between p-HTMOPPCu (II) and c-myc G-quadruplex DNA. Results: The UV absorption spectrum and fluorescence spectroscopy results indicated that the complex of p-HTMOPPCu (II) bound better with c-myc G-quadruplex DNA; the FRET melting point assay, and competitive melting point assay demonstrated that p-HTMOPPCu (II) could selectively bind and stabilize c-myc G-quadruplex DNA. Conclusion: p-HTMOPPCu (II) can bind and stabilize c-myc G-quadruplex DNA and will potentially be developed into a class of small molecule inhibitors targeting c-myc G-quadruplex DNA for clinical applications in the treatment of tumors. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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