| Autor: |
Maksim V. Baranov, Melina Ioannidis, Sami Balahsioui, Auke Boersma, Rinse de Boer, Manoj Kumar, Masato Niwa, Tasuku Hirayama, Qintian Zhou, Terrence M. Hopkins, Pieter Grijpstra, Shashi Thutupalli, Stefano Sacanna, Geert van den Bogaart |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Biophysical Reports, Vol 2, Iss 3, Pp 100069- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2667-0747 |
| DOI: |
10.1016/j.bpr.2022.100069 |
| Popis: |
Localized fluxes, production, and/or degradation coupled to limited diffusion are well known to result in stable spatial concentration gradients of biomolecules in the cell. In this study, we demonstrate that this also holds true for small ions, since we found that the close membrane apposition between the membrane of a phagosome and the surface of the cargo particle it encloses, together with localized membrane rupture, suffice for stable gradients of protons and iron cations within the lumen of the phagosome. Our data show that, in phagosomes containing hexapod-shaped silica colloid particles, the phagosomal membrane is ruptured at the positions of the tips of the rods, but not at other positions. This results in the confined leakage at these positions of protons and iron from the lumen of the phagosome into the cytosol. In contrast, acidification and iron accumulation still occur at the positions of the phagosomes nearer to the cores of the particles. Our study strengthens the concept that coupling metabolic and signaling reaction cascades can be spatially confined by localized limited diffusion. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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