Higher induction of defense enzymes and cell wall reinforcement in maize by root associated bacteria for better protection against Aspergillus niger

Autor: Yachana Jha
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Journal of Plant Protection Research, Vol 59, Iss 3, Pp 341-349 (2019)
Druh dokumentu: article
ISSN: 1899-007X
DOI: 10.24425/jppr.2019.129746
Popis: Root associated bacteria were isolated from Suaeda nudiflora and two isolates were selected for this study: rhizospheric Bacillus megaterium and endophytic Pseudomonas aeruginosa. These isolates were inoculated into maize variety Narmada Moti during its germination. TTC (2, 3, 5-triphenyl tetrazolium chloride) staining was used to confirm the association of the isolates with the maize root. The effects of these root associated bacteria were tested alone and in combinations for cell wall reinforcement and the induction of defense enzymes such as phenylalanine ammonia lyase (PAL) and β-1,3-glucanase in the presence of fungal pathogen Aspergillus niger in maize. The results indicated that the rhizospheric bacteria had a greater fight response to fungal infection than the endophhytic bacteria due to cell wall lignification as well as the rapid induction of higher concentrations of defense related enzymes.
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