The cofactor-dependent folding mechanism of Drosophila cryptochrome revealed by single-molecule pulling experiments

Autor: Sahar Foroutannejad, Lydia L. Good, Changfan Lin, Zachariah I. Carter, Mahlet G. Tadesse, Aaron L. Lucius, Brian R. Crane, Rodrigo A. Maillard
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-023-36701-y
Popis: Characterizing folding pathways of large proteins that bind complex cofactors is challenging. The authors use optical tweezers to study the 542-residue FAD-binding lightsensor protein dCRY, identifying several intermediates and cofactor binding steps, and dissecting the role of FAD moieties in folding.
Databáze: Directory of Open Access Journals