LIGHT EXPOSURE’S EFFECTS ON INACTIVE STATE DURATION AND SLEEP LATENCY IN ZEBRAFISH (DANIO RERIO) LARVAE INSOMNIA MODEL
| Autor: | Zamroni Afif, Mochammad Istiadjid Eddy Santoso, Husnul Khotimah, Irawan Satriotomo, Edi Widjajanto, Masruroh Rahayu, Shahdevi Nandar Kurniawan, Dheka Sapti Iskandar, Annisatul Hakimah, Syafiatul Azizah, Nurvia Andriani, Kartika Agustina |
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| Jazyk: | English<br />Indonesian |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Malang Neurology Journal, Vol 8, Iss 2, Pp 129-134 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2407-6724 2442-5001 |
| DOI: | 10.21776/ub.mnj.2022.008.02.11 |
| Popis: | Background: Insomnia is defined as difficulty falling or staying asleep or a sleep state that cannot restore the body's condition. The zebrafish (Danio rerio) is a vertebrate model that has been extensively studied to study sleep and neurological disorders. One of the most widely used methods to examine the effect of the light-dark cycle on the circadian system is by exposing animals and humans to pulse wave light. Objective: To see the effect of light exposure on zebrafish larvae by looking at inactive state duration and sleep latency in zebrafish (Danio rerio) larvae insomnia model. Methods: This study used four groups of zebrafish larvae i.e : (1) normal group (2) minutes of light exposure and 2 minutes off (2/2)), (3) Four minutes of light exposure and 1 minute off (4/1), and (4) 24 hours on (24/0). Observation of larval movement was carried out on 5, 6, and 7 dpf (day post fertilization). Observation time was 30 minutes before and after turned off of light exposure. Results: There were significant differences on days 5, 6, and 7 between the normal group and the three light treatment groups on inactive state duration and sleep latency in dark and light conditions with p-values (p |
| Databáze: | Directory of Open Access Journals |
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