Apoptosis of porcine Sertoli cells is inhibited by QKI-5 via regulating CASP8
| Autor: | Mengdi Liang, Xin Liu, Jia Guo, Yuwei Yang, Yonghong Zhang, Xianzhong Yu, Boxing Sun |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Czech Journal of Animal Science, Vol 64, Iss 5, Pp 207-215 (2019) |
| Druh dokumentu: | article |
| ISSN: | 1212-1819 1805-9309 |
| DOI: | 10.17221/158/2018-CJAS |
| Popis: | QKI, a KH domain containing RNA binding, is an RNA-binding protein that is involved in cell proliferation and apoptosis through binding to the QKI response element (QRE) site of its target mRNA. And Caspase 8 (CASP8) and Caspase 3 (CASP3) play important roles in the pathway of apoptosis. The purpose of this study was to investigate the effect of QKI-5 on the apoptosis of Sertoli cells. The experimental results show that pig tissues contain QKI-5, QKI-6 and QKI-7. Overexpression of QKI-5 significantly decreased the mRNA expression of CASP8 (P < 0.05) and the protein expression of CASP8 (P < 0.05). On the contrary, inhibiting QKI-5 increased the expression of CASP8 significantly. Overexpression of QKI-5 significantly reduced the apoptosis of Sertoli cells and promoted cell growth (P < 0.05). Furthermore, QKI-5 specifically reduced the stability of CASP8 mRNA by binding QRE sites on CASP8. Our experiments provide preliminary evidence that QKI-5 induces Sertoli cells proliferation by inhibiting apoptosis, and this may be one of the factors promoting testicular development. |
| Databáze: | Directory of Open Access Journals |
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