| Autor: |
Nhlamulo G. Sibiya, Ditebogo M. Mamabolo, Cara Greyling, Evans M. N. Chirwa, Michael Daramola, Sekgetho C. Mokwatlo, Hendrik G. Brink |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Chemical Engineering Transactions, Vol 94 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2283-9216 |
| DOI: |
10.3303/CET2294239 |
| Popis: |
The current study focused on using a previously published model known to accurately predict the cultivation of Actinobacillus succionogenes on glucose in a NaOH neutralised system, for the prediction of batch system behaviour when MgCO3 is used as a pH buffer in a batch reactor system. The use of MgCO3 as a neutralising agent resulted in a biomass growth lag of about 8 h, followed by a sharp increase untill 15 h at which point the biomass concentration remained constant throughout the fermentation run. The maximum biomass concentration was 3.62 g/L. The fermentation ceased at a final SA concentration of 38.12 g/L and the SA yield on glucose was 0.81 g/g from an initial glucose concentration of 60 g/L. At the end of the fermentation, 79 % of the glucose as consumed. A residual glucose concentration of 12.84 g/L at the maximum SA concentration indicated product-related inhibition. The biomass, acetic acid (AA), and formic acid (FA) concentrations were incorrectly predicted by the model which implies that the use of MgCO3 as a buffer agent changed the metabolic fluxes of the organism. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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