Localization of urea transporter B in the developing bovine rumen

Autor: Chongliang Zhong, Tamsin Lyons, Orla Heussaff, Evelyn Doyle, Eoin O'Hara, Sinead M. Waters, David Kenny, Gavin S. Stewart
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Animal Nutrition, Vol 10, Iss , Pp 216-222 (2022)
Druh dokumentu: article
ISSN: 2405-6545
DOI: 10.1016/j.aninu.2022.03.006
Popis: Urea nitrogen secreted from blood to rumen is a crucial factor shaping the symbiotic relationship between host ruminants and their microbial populations. Passage of urea across rumen epithelia is facilitated by urea transporter B (UT-B), but the long-term regulation of these proteins remains unclear. As ruminal function develops over a period of months, the developing rumen is an excellent model with which to investigate this regulation. Using rumen epithelium samples of calves from birth to 96 d of age, this study performed immunolocalization studies to localize and semi-quantify UT-B protein development. As expected, preliminary experiments confirmed that ruminal monocarboxylate transporter 1 (MCT1) short chain fatty acid transporter protein abundance increased with age (P
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