Development and validation of a High Performance Liquid Chromatographic method for determination of etoposide in biodegradable polymeric implants
| Autor: | Ana Gabriela Reis Solano, Gisele Rodrigues da Silva, Silvia Ligório Fialho, Armando da Silva Cunha Júnior, Gérson Antônio Pianetti |
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| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 35, Iss 6, Pp 1239-1243 (2012) |
| Druh dokumentu: | article |
| ISSN: | 1678-7064 0100-4042 |
| DOI: | 10.1590/S0100-40422012000600032 |
| Popis: | A method using HPLC-UV was developed and validated for the determination of etoposide incorporated into polycaprolactone implants. The method was carried out in isocratic mode using a C18 column (250 x 4.6 mm; 5 µm), at 25 ºC, with acetonitrile and acetic acid 4% (70:30) as mobile phase, a flow rate of 2 mL/min, and UV detection at 285 nm. The method was linear (r² > 0.99) over the range of 5 to 65 µg/mL, precise (RSD < 5%), accurate (recovery of 98.7%), robust, selective regarding excipient of the sample, and had a quantitation limit equal to 1.76 µg/mL. The validated method can be successfully employed for routine quality control analyses. |
| Databáze: | Directory of Open Access Journals |
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