Um experimento de análise em fluxo envolvendo reações enzimáticas e quimiluminescência
| Autor: | Leite Oldair Donizeti, Fatibello-Filho Orlando, Rocha Fábio R. P. |
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| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2004 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 27, Iss 2, Pp 337-341 (2004) |
| Druh dokumentu: | article |
| ISSN: | 0100-4042 1678-7064 |
| Popis: | An experiment is proposed to introduce some fundamentals of flow analysis, chemiluminescence and kinetic monitoring of enzymatic reactions in undergraduate courses. Chemiluminescence detection is performed with a simple spectrophotometer equipped with a lab-made spiral flow cell constructed from a polyethylene tube. The hydrogen peroxide produced by the glucose oxidation in the presence of glucose oxidase is continuously monitored by the reaction with luminol in alkaline media in a flow injection system. The exercise allows also the discussion of important analytical features and the comparison with different optical methods of analysis. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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