Mycobacterium bovis: polymerase chain reaction identification in bovine Lymphonode biopsies and genotyping in isolates from Southeast Brazil by spolygotyping and restriction fragment length polymorphism
| Autor: | Zanini MS, Moreira EC, Lopes MTP, Oliveira RS, Leão SC, Fioravanti RL, Roxo E, Zumarraga M, Romano MI, Cataldi A, Salas CE |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | Memorias do Instituto Oswaldo Cruz, Vol 96, Iss 6, Pp 809-813 (2001) |
| Druh dokumentu: | article |
| ISSN: | 0074-0276 1678-8060 |
| Popis: | Diagnosis of the Mycobacterium tuberculosis complex by direct PCR of mediastinal lymphnode DNA and microbiological tests were compared in cattle suspicious of bearing tuberculous-like lesions detected during slaughter. The PCR procedure applied on DNA samples (n=54) obtained by adding alpha -casein into the thiocyanate extraction mix was positive in 70% of the samples. PCR confirmed the identification of 23 samples (100%) that grew in culture, 9 samples (60%) that failed to grow in culture, plus 6 (37.5%) samples that resulted in growth of bacterial contaminants. Genotyping by IS6110-RFLP and DR-spoligotyping analysis of seven samples revealed the presence of several polimorphisms. Seven of the isolates contained multiple copies of IS6110, thus defining the existence of five singular genotypes. |
| Databáze: | Directory of Open Access Journals |
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