| Autor: |
Hwee-Leng Siow, Sy-Bing Choi, Chee-Yuen Gan |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Journal of Functional Foods, Vol 27, Iss , Pp 600-611 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
1756-4646 |
| DOI: |
10.1016/j.jff.2016.10.013 |
| Popis: |
The objective of this study was to evaluate the in vitro physiological properties of pre-screened cumin seed peptides (CSPs) based on in vitro and in silico studies. Results showed that CSPs were capable of increasing the protein digestibility up to 400%. Apart from that, CSP1 and CSP2 showed >50% inhibition of pancreatic lipase activity. These peptides also exerted a similar or greater affinity to bind bile acid than cholestyramine, in addition to giving inhibitory effect (up to 80%) in the formation of cholesterol micelle. Based on the structure–activity relationship studies, the results have postulated that the interaction of peptides at non-catalytic region induced allosteric effects to enhance the pepsin proteolytic activity; the direct contact of the peptide with lipase active sites rendered inhibitory action; and the binding between peptide and bile acids was relied on the hydrophilic and hydrophobic interactions. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect