| Autor: |
Matvey S. Pochechuev, Ilya V. Fedotov, Maxim A. Solotenkov, Maria S. Andreeva, Aleksandr A. Lanin, Andrei B. Fedotov, Aleksei M. Zheltikov |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Photonics, Vol 9, Iss 1, p 21 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2304-6732 |
| DOI: |
10.3390/photonics9010021 |
| Popis: |
We demonstrate an adaptive wave-front shaping of optical beams transmitted through fiber bundles as a powerful resource for multisite, high-resolution bioimaging. With the phases of all the beamlets delivered through up to 6000 different fibers within the fiber bundle controlled individually, by means of a high-definition spatial light modulator, the overall beam transmitted through the fiber bundle can be focused into a beam waist with a diameter less than 1 μm within a targeted area in a biotissue, providing a diffraction-limited spatial resolution adequate for single-cell or even subcellular bioimaging. The field intensity in the adaptively-focused continuous-wave laser beam in our fiber-bundle-imaging setting is more than two orders of magnitude higher than the intensity of the speckle background. Once robust beam focusing was achieved with a suitable phase profile across the input face of the fiber bundle, the beam focus can be scanned over a targeted area with no need for a further adaptive search, by applying a physically intuitive, wave-front-tilting phase mask on the field of input beamlets. This method of beam-focus scanning promises imaging speeds compatible with the requirements of in vivo calcium imaging. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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