Hematobiochemical alterations and direct blood polymerase chain reaction detection of Theileria annulata in naturally infected crossbred cows

Autor: Anita Ganguly, Vandna Bhanot, R. S. Bisla, Indrajit Ganguly, Harpreet Singh, S. S. Chaudhri
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Veterinary World, Vol 8, Iss 1, Pp 24-28 (2015)
Druh dokumentu: article
ISSN: 0972-8988
2231-0916
DOI: 10.14202/vetworld.2015.24-28
Popis: Aim: The aim was to determine hemato-biochemical changes and rapid diagnosis of Theileria annulata in naturally infected crossbred cows. Materials and Methods: Blood samples from lactating crossbred cows (n=40) between 3 and 7 years of age and showing clinical signs of tropical theileriosis were collected, with or without anticoagulant, and analyzed for tropical theileriosis by direct smear, direct blood polymerase chain reaction (PCR) detection of merozoite-piroplasm surface antigen (Tams1) gene specific amplicon, estimation of hematological and biochemical parameters. Healthy crossbred cows (n=6), examined free from hemoprotozoan infections were included as control. Results: The infected crossbred cows revealed significantly (p
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