| Autor: |
Olga Pacios, Soraya Herrera-Espejo, Lucía Armán, Clara Ibarguren-Quiles, Lucía Blasco, Inés Bleriot, Laura Fernández-García, Concha Ortiz-Cartagena, María Paniagua, Antonio Barrio-Pujante, Belén Aracil, José Miguel Cisneros, María Eugenia Pachón-Ibáñez, María Tomás |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Antibiotics, Vol 13, Iss 9, p 815 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2079-6382 |
| DOI: |
10.3390/antibiotics13090815 |
| Popis: |
The combination of several therapeutic strategies is often seen as a good way to decrease resistance rates, since bacteria can more easily overcome single-drug treatments than multi-drug ones. This strategy is especially attractive when several targets and subpopulations are affected, as it is the case of Klebsiella pneumoniae persister cells, a subpopulation of bacteria able to transiently survive antibiotic exposures. This work aims to evaluate the potential of a repurposed anticancer drug, mitomycin C, combined with the K. pneumoniae lytic phage vB_KpnM-VAC13 in vitro and its safety in an in vivo murine model against two clinical isolates of this pathogen, one of them exhibiting an imipenem-persister phenotype. At the same time, we verified the absence of toxicity of mitomycin C at the concentration using the human chondrocyte cell line T/C28a2. The viability of these human cells was checked using both cytotoxicity assays and flow cytometry. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
