The retaining β-Kdo glycosyltransferase WbbB uses a double-displacement mechanism with an intermediate adduct rearrangement step

Autor: Taylor J. B. Forrester, Olga G. Ovchinnikova, Zhixiong Li, Elena N. Kitova, Jeremy T. Nothof, Akihiko Koizumi, John S. Klassen, Todd L. Lowary, Chris Whitfield, Matthew S. Kimber
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-022-33988-1
Popis: WbbB is a structurally unusual retaining glycosyltransferase. Here, the authors show that WbbB forms an Asp232-Kdo adduct prior to transfer to the saccharide acceptor. Therefore, unlike any previously studied glycosyltransferase, WbbB uses the double-displacement mechanism first proposed in 1953.
Databáze: Directory of Open Access Journals