Autor: |
Xiaozhou Zhang, Xiaojian Zhang, Qian Jia, Hongqiang Li, Runsheng Ma, Guang Yang, Fengyan Yin, Nannan Jiang, Detao Yin |
Jazyk: |
angličtina |
Rok vydání: |
2022 |
Předmět: |
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Zdroj: |
BMC Cancer, Vol 22, Iss 1, Pp 1-11 (2022) |
Druh dokumentu: |
article |
ISSN: |
1471-2407 |
DOI: |
10.1186/s12885-022-09360-3 |
Popis: |
Abstract Background The incidence and mortality of thyroid cancer (TC) has been steadily rising in the past decades. It is imperative to have a better understanding of the molecular mechanisms underlying TC development and identify novel therapeutic targets. This study characterized the role of lncRNA CALML3-AS1 (CALML3-AS1) in the development of papillary thyroid cancer (PTC). Method Related mRNAs expression were validated in the tumor and adjacent normal tissues from 52 PTC patients and PTC cell lines by qRT-PCR. Expression of RBM38 was detected by Western blot. We have also conducted CCK-8 and colony formation assays were used to detect the effect of CALML3-AS1 on cell proliferation, Transwell assay was utilized to evaluate cell migration and invasion, apoptosis detected by flow cytometry assay, RNA pull-down and luciferase assays were performed to validate gene predictions. Results The results indicated that the expression of both CALML3A-S1 and RBM38 were significantly downregulated in PTC tissues (p |
Databáze: |
Directory of Open Access Journals |
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