Keragaman genetik mimi (Carcinoscorpius rotundicauda dan Tachypleus gigas) di perairan Demak, Madura dan Balikpapan berdasarkan penanda Random Amplified Polymorphic DNA

Autor: NAILA KHURIL AINI, ALI MASHAR, Hawis H. MADDUPPA, YUSLI WARDIATNO
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Journal of Natural Resources and Environmental Management, Vol 10, Iss 1, Pp 124-137 (2020)
Druh dokumentu: article
ISSN: 2086-4639
2460-5824
DOI: 10.29244/jpsl.10.1.124-137
Popis: Horseshoe crab is an exotic and protected marine organism in Indonesia and is considered as the living fossil animal in the world. IUCN conservation status of Carcinoscorpius rotundicauda and Tachypleus gigas is still Data Deficient, and in Indonesia research on genetic population is lacking, if any. This study aims to reveal genetic diversity of C. rotundicauda and T. gigas populations in Demak, Madura, and Balikpapan coastal waters. This research was conducted by using molecular analysis with genetic markers Random Amplified Polymorphic DNA (RAPD). The primers used in this study were OPB 06, OPG 10, and OPX 03. The results showed that the highest polymorphism of C. rotundicauda was found in Demak (74.6667%) and heterozygosity was 0.2669. Furthermore, T. gigas had the highest polymorphism in Madura (74.3590%) and heterozygosity was 0.25551. Based on pairwise comparison tests, populations of C. rotundicauda and T. gigas in Demak, Madura, and Balikpapan were significantly different (p
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