In vivo imaging of prostate tumor-targeted folic acid conjugated quantum dots

Autor: Sulaxna Pandey, Prakash Choudhary, Virendra Gajbhiye, Sachin Jadhav, Dhananjay Bodas
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Cancer Nanotechnology, Vol 14, Iss 1, Pp 1-11 (2023)
Druh dokumentu: article
ISSN: 1868-6958
1868-6966
DOI: 10.1186/s12645-023-00162-1
Popis: Abstract Cancer is a major threat to human health; thus, early detection is imperative for successful management. Rapid diagnosis can be achieved by imaging primary (subcutaneous) tumors using fluorophores conjugated with tumor markers. Here, the application of biocompatible, quantum efficient, monodisperse, and photostable polymer-coated quantum dots (PQDs) is demonstrated for targeted prostate tumor imaging in living SCID mice. Briefly, PQDs (blue) are conjugated to folic acid (FA-PQDs) using DCC-NHS chemistry. Initially, in vitro targeted imaging via FA-PQDs is evaluated in LNCaP cells. The confocal microscopic evaluation demonstrates the uptake of FA-PQDs. To understand the dispersion of PQDs in vivo, the biodistribution of PQDs is assessed at different time intervals (1- 180 min) using whole-body fluorescence imaging and computed tomography (CT) scan. PQDs are seen to accumulate in organs like the liver, kidneys, spleen, lungs, and urinary bladder within 60 min, however, PQDs are not observed at 180 min indicating renal clearance. Further, to target the prostate tumor (~ 200 mm3) in mice, FA-PQDs are injected intravenously, and whole-body fluorescence imaging along with a CT scan is recorded. FA-PQDs are seen at the tumor site as compared to PQDs. The results confirm that the FA-PQDs function as excellent nanoprobes for targeted tumor imaging in vivo.
Databáze: Directory of Open Access Journals
Externí odkaz: