| Autor: |
Michelle de Paula Farias, Felipe de Souza Matos, Nayane Chagas Carvalho, Roque Pacheco de Almeida, Adriano Augusto Melo de Mendonça, Ricardo Luiz Cavalcanti de Albuquerque Júnior, Maria Amália Gonzaga Ribeiro |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Bioscience Journal, Vol 32, Iss 2 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
1981-3163 |
| DOI: |
10.14393/BJ-v32n2a2016-30974 |
| Popis: |
This study aimed to evaluate the cytotoxicity of intracanal medications on L929 fibroblast cells at different periods of observation. The following experimental groups were studied: calcium hydroxide with camphorated paramonochlorophenol and glycerin (CPG); iodoform with glycerin (IG); calcium hydroxide with iodoform and distilled water (CIW); iodoform with distilled water (IW); calcium hydroxide with distilled water (CW); Otosporin® (OT); and a control group composed of cells and culture medium. Eluates were prepared from each group and placed in contact with 1 x 105 cells/well for periods of 30 minutes, 12, 24, 48 and 72 hours, 5 and 7 days. After each experimental period, a cytotoxicity test was performed using methyltetrazolium (MTT) and a spectrophotometer at an optical density of 570 nm to analyze cell viability. The ANOVA and Tukey test with a significance level of 5% was used to analyze the data. At 30 minutes and at 12 hours, all groups were equal to the control group. At 24 hours, there was greater cytotoxicity in the IG group than in the control group (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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