Platelet-derived growth factor-C functions as a growth factor in mouse embryonic stem cells and human fibrosarcoma cells

Autor: Tomoaki Kinjo, Chuanhai Sun, Tomomi Ikeda, Takako Ikegami, Yuhki Tada, Tadayuki Akagi, Takashi Yokota, Hiroshi Koide
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Cellular & Molecular Biology Letters, Vol 23, Iss 1, Pp 1-11 (2018)
Druh dokumentu: article
ISSN: 1425-8153
1689-1392
DOI: 10.1186/s11658-018-0075-3
Popis: Abstract Background Platelet-derived growth factor-C (PDGF-C) has been shown to be involved in several biological processes, such as embryonic development, wound healing and angiogenesis, as well as in diseases including tumor formation and fibrotic diseases. However, its role in fibrosarcoma and embryonic stem (ES) cells has not been elucidated. Methods The expression level of PDGF-C was measured using RT-PCR. The activity of PDGF-C was suppressed using RNA interference or a neutralizing antibody and the effect on cell growth was examined using the WST and soft agar assays. Results In the tumor cell lines studied, the highest level of PDGF-C expression was in human HT1080 fibrosarcoma cells. In ES cells, it was highly expressed in the self-renewal state but not in the differentiated state. PDGF-C knockdown suppressed anchorage-dependent and -independent growth of HT1080 and ES cells. In addition, the suppression of PDGF-C activity by a neutralizing antibody retarded ES cell growth. Conclusion Our results suggest that PDGF-C plays an important role in the proliferation of fibrosarcoma and ES cells.
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