| Autor: |
Lydia-Ann L.S. Harris, Trevor M. Shew, James R. Skinner, Nathan E. Wolins |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 53, Iss 5, Pp 1021-1025 (2012) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1194/jlr.D023598 |
| Popis: |
Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tissue are fractionated with a single centrifugation step in a standard microcentrifuge. This procedure reproducibly yields three fractions highly enriched in either FDs, soluble cellular components, or sedimentable organelles/membranes. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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