| Autor: |
Joel B. Johnson, Ryan J. Batley, Janice S. Mani, Mani Naiker |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Engineering Proceedings, Vol 56, Iss 1, p 80 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2673-4591 |
| DOI: |
10.3390/ASEC2023-15407 |
| Popis: |
This proof-of-concept study demonstrated the potential of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy for the structural characterization of natural products when only very small quantities of the target compound are available. Four known compounds (6-gingerol, 6-shogaol, 8-gingerol and 10-gingerol) were isolated from ginger (Zingiber officinale) rhizome using semi-preparative high-performance liquid chromatography (HPLC). A portion of each fraction was evaporated on the ATR plate and spectra collected using a standard FTIR instrument. The minimum amount required to detect some spectral features appeared to be around 50 ng for the gingerols, and around 25 ng for 6-shogaol. Various peaks are assigned and interpreted to demonstrate the range of structural information that can be obtained. Evaporation-based ATR-FTIR spectroscopy could be an inexpensive and rapid method to aid structural elucidation of natural compounds, even when collected from a single semi-preparative HPLC run. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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