| Autor: |
Tove Selvin, Malin Berglund, Anders Åkerström, Marco Zia, Jakob Rudfeldt, Malin Jarvius, Rolf Larsson, Claes R Andersson, Mårten Fryknäs |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
SLAS Technology, Vol 29, Iss 6, Pp 100232- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2472-6303 |
| DOI: |
10.1016/j.slast.2024.100232 |
| Popis: |
To facilitate the translation of immunotherapies from bench to bedside, predictive preclinical models are essential. We developed the in vivo immuno-oncology Hollow Fiber Assay (HFA) to bridge the gap between simpler cell-based in vitro assays and more complex mouse models for immuno-oncology drug evaluation. The assay involves co-culturing human cancer cell lines or primary patient-derived cancer cells with human immune cells inside semipermeable hollow fibers. Implanted intraperitoneally in mice, the fibers captured treatment-induced immune cell-mediated cancer cell killing following treatments with aCD3 and/or IL-2, demonstrating the feasibility of the assay. Traditional models require lengthy observation periods to monitor tumor growth and treatment response. The immuno-oncology HFA enables a rapid initial in vivo evaluation of immunological agents on cancer and immune cells of human origin, addressing two of the 3Rs — reduction and refinement — in animal research. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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