| Autor: |
Terrence Ravine, Qunying Yuan, Makenna Howell |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Applied Nano, Vol 3, Iss 4, Pp 187-201 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2673-3501 |
| DOI: |
10.3390/applnano3040014 |
| Popis: |
Biogenic silver nanoparticles (b-AgNPs) were produced extracellularly using a cell lysate of genetically modified Escherichia coli and subdivided into three groups. Each group received a different treatment to determine which one best removed residual cell lysate material. The first group was treated twice using only water (water ×2), the second using 8M urea once (8M urea ×1), and the third using 8M urea twice (8M urea ×2). Subsequently, each group was assessed for its ability to inhibit the growth of six bacterial and two fungal pathogens. Testing was accomplished using the minimum inhibitory concentration (MIC) method. Commercially produced c-AgNPs were included for comparison. In all cases, the b-AgNPs (8M urea ×2) demonstrated the greatest inhibition of microbe growth. Conversely, the commercial AgNPs failed to show any growth inhibition at 10 µg/mL the highest concentration tested. The greater antibacterial activity of the b-AgNPs (8M urea ×2) over both b-AgNPs (8M urea ×1) and b-AgNPs (water ×2) is thought to be due to a larger degree of biofunctionalization (coating) occurring during the two sequential 8M urea treatments. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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